proteome profiler mouse cytokine array kit  (R&D Systems)

Journal: International Journal of Molecular Sciences

Article Title: Polarized Anti-Inflammatory Mesenchymal Stem Cells Increase Hippocampal Neurogenesis and Improve Cognitive Function in Aged Mice

doi: 10.3390/ijms24054490

Figure Lengend Snippet: PACAP polarizes MSC towards an anti-inflammatory (MSC2) phenotype. Conditioned medium from naïve and PACAP-treated MSC was analyzed for chemokines and cytokines secretion, utilizing Proteome Profiler Mouse Cytokine Array (R&D Systems). The following chemokine and anti-inflammatory cytokines IL-2, IL-3, IL-4, IL-27, IP10, IL-1ra, RANTES, SDF-1, CCL2(JE), CCL1 (i-309), G-CSF, BLC were upregulated, while pro-inflammatory cytokines were downregulated (IL-6, IL-1a, IFN-ɣ and soluble ICAM-1). Analysis was performed on pooled samples (n = 3) for each culture.

Article Snippet: Cytokine expression was detected in 100 μL of serum or pooled conditioned media using the Proteome Profiler Mouse Cytokine Array Kit (R&D Systems, Cat no. SC018), according to the manufacturer’s protocol.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Polarized Anti-Inflammatory Mesenchymal Stem Cells Increase Hippocampal Neurogenesis and Improve Cognitive Function in Aged Mice

doi: 10.3390/ijms24054490

Figure Lengend Snippet: Systemic administration of polarized MSC to aged mice normalizes systemic chemokine levels. Intravenous injection of polarized anti-inflammatory MSC (pMSC, N = 3–4) reduces the levels of plasma chemokines that are associated with aging and inflammation to levels of young (3 months old) mice (N = 7–8) compared with vehicle-treated aged control mice (N = 3), as detected by Proteome Profiler Mouse Cytokine Array (R&D Systems). Bars in the graph present mean ± SE. * p < 0.05, ** p < 0.01. One-way ANOVA. ns = non-significant.

Article Snippet: Cytokine expression was detected in 100 μL of serum or pooled conditioned media using the Proteome Profiler Mouse Cytokine Array Kit (R&D Systems, Cat no. SC018), according to the manufacturer’s protocol.

Techniques: Injection, Clinical Proteomics, Control

Journal: Stem cells and development

Article Title: Human Cardiac-Mesenchymal Stem Cell-Like Cells, a Novel Cell Population with Therapeutic Potential.

doi: 10.1089/scd.2018.0170

Figure Lengend Snippet: FIG. 1. Morphology of different primary cells in culture. Representative brightfield images of cells in vitro at passage 3. (A) BM-derived MSCs, (B–D) show images from three different patient-derived cul- tures of CMSCLC. Brightfield image showing morphology of human CDCs (E) Brightfield image of CMSCLC CFU (F). All scale bars =100mm. CMSCLC cultures derived from three different patient tissue samples were assessed for their ability to form CFUs under standard MSC culture conditions, and BM was also assessed for CFU formation ability (G). The number of CFUs formed per culture at passage 1 is represented graphically. MSCs, mesenchy- mal stem cells; CMSCLC, cardiac-derived mesenchymal stem cell-like cells; BM, bone marrow. Color images are available online.

Article Snippet: Primary antibodies and IgG control were taken from the Human Multipotent Mesenchymal Stromal Cell Marker Antibody Panel kit (R&D Systems).

Techniques: In Vitro, Derivative Assay